![]() The CliniMACS Prodigy device, tubing set TS520 and TCT software allow CAR-T cells to be manufactured in a closed system at the treatment site without need for clean-room facilities and related infrastructure.ĬliniMACS Prodigy chimeric antigen receptor T cells immunotherapy lentiviral vectors.Ĭopyright © 2017 International Society for Cellular Therapy. Functional testing demonstrated recognition of B-cells and for the CAR-20/19 T cells, CD19 and CD20 single transfectants were recognized in cytotoxic T lymphocyte and interferon-γ production assays. CAR-T cells were 82-100% CD3 + with a mix of CD4 + and CD8 + cells that primarily expressed an effector-memory or central-memory phenotype. An average 30-fold expansion of 10 8 CD4/CD8-enriched cells resulted in sufficient transduced T cells for clinical use. 2303 Lindbergh Street Auburn, CA 95602-9562, USA Phone 800 FOR MACS Phone +1 5 Fax +18 Miltenyi Biotec Australia Pty. It is manufactured without animal-derived components. This complete medium enables reproducible application in human and mouse cell culture. Vectors at multiplicity of infection 5-10 resulted in transduction averaging 37%. TexMACS Medium is an optimized serum-free cell culture medium developed for the cultivation and expansion of human and mouse T cells and regulatory T cells. Processing time was 13 days.Įnrichment (N = 7) resulted in CD4/CD8 purity of 98 ± 4.0%, 55 ± 6% recovery and CD3 + T-cell purity of 89 ± 10%. We used MACS-CD4 and CD8-MicroBeads for enrichment, TransAct CD3/CD28 reagent for activation, lentiviral CD8 TM-41BB-CD3 ζ-cfrag vectors expressing scFv for CD19 or CD20/CD19 antigens for transduction, TexMACS medium-3%-HS-IL2 for culture and phosphate-buffered saline/ethylenediaminetetraacetic acid buffer for washing. The CliniMACS Prodigy with TCT process software and the TS520 tubing set that allows closed-system processing for cell enrichment, transduction, washing and expansion was used. Recently, Miltenyi Biotec has allowed the automated clinical-scale. Simplified, reproducible CAR-T-cell manufacturing with reduced labor intensity within a closed-system is highly desirable for increased availability for patients. Results: NK MACS and TexMACs achieved the highest NK cell purity and lowest T cell. This complex process requires skilled personnel and costly clean-room facilities and infrastructure. ![]() ![]() Bioz Stars score: 86/100, based on 1 PubMed citations. ![]() Open processing steps that increase risk of contamination and production failure are required. Miltenyi Biotec cd25 texmacs expact t reg kit Cd25 Texmacs Expact T Reg Kit, supplied by Miltenyi Biotec, used in various techniques. Multiple steps are required to produce chimeric antigen receptor (CAR)-T cells, involving subset enrichment or depletion, activation, gene transduction and expansion. ![]()
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